Comparative effects of proprioceptive neuromuscular facilitation hold-relax and dynamic stretching on hamstring flexibility, sprint performance, and perceived exertion in adolescent sprinters

Authors

Keywords:

athletes, muscular, sprint performance, static, strength

Abstract

Background and Study Aim. Stretching is commonly used to support physical performance and preparation in sprint training. Different approaches are applied to influence muscle function and readiness for activity. The study investigated the comparative efficacy of proprioceptive neuromuscular facilitation hold-relax (PNF-HR) and dynamic stretching (DS) on hamstring flexibility, sprint performance, and perceived exertion in adolescent male sprinters. Materials and Methods. The study included eighty-four adolescent male sprinters (aged 15–18 years) recruited from three training academies in Lahore. Participants were randomly allocated to the PNF-HR group, DS group, or control group. The experimental groups completed three supervised sessions per week. Hamstring flexibility was assessed using the Active Knee Extension Test. Sprint performance was assessed using 30 m dash times. Perceived exertion was assessed using the Borg CR-10 scale. Data were collected at baseline, after four weeks, and after eight weeks. Results. Mixed-model ANOVA showed significant time × group interactions for all primary outcomes (p < .001). These results indicate differential changes between groups over time. Ninety-five percent confidence intervals and effect sizes were calculated for the primary outcomes. Post hoc analysis showed greater improvement in flexibility in the PNF-HR group (Δ 16.2°, d = 2.14) compared with the DS group (Δ 9.8°, d = 1.31) and the control group. Both PNF-HR and DS improved 30 m sprint time. The DS group showed a greater reduction (-0.21 s) compared with the PNF-HR group (-0.14 s). Conclusions. Perceived exertion was higher in the PNF-HR group (mean = 7.4) than in the DS group (mean = 4.1). The PNF-HR method improved flexibility. The DS modality was associated with better sprint performance and lower perceived effort. A targeted stretching modality should be selected based on the training goal in adolescent athletes. Future studies should examine long-term injury rates and the combined use of these modalities within training programs.

Author Biographies

Mohibullah Khan Marwat, The University of Lahore

Professor; mohibullah.khan@ed.uol.edu.pk; Department of Sports Sciences and Physical Education, Faculty of Allied Health Sciences; Lahore, Pakistan.

Yasmeen Iqbal, The University of Lahore

Professor; yasmeen.iqbal@ahs.uol.edu.pk; Department of Sports Sciences and Physical Education, Faculty of Allied Health Sciences; Lahore, Pakistan.

Syed Muhammad Bilal Gillani, The University of Lahore

Assistan Professor; muhammad.bilal@sps.uol.edu.pk; Department of Sports Sciences and Physical Education, Faculty of Allied Health Sciences; Lahore, Pakistan.

Mufeez Alam, The University of Lahore

mufeez.alam@ahs.uol.edu.pk; Department of Sports Sciences and Physical Education, Faculty of Allied Health Sciences; Lahore, Pakistan.

Muhammad Salman, The University of Lahore

drsalman705@gmail.com; Department of Sports Sciences and Physical Education, Faculty of Allied Health Sciences; Lahore, Pakistan.

Kashif Mehmood, The University of Lahore

kashif.mehmood@sports.uol.edu.pk; Department of Sports Sciences and Physical Education, Faculty of Allied Health Sciences; Lahore, Pakistan.

Muhammad Asad Khan, Ayub Medical College, Abbott Abad

asad.mrwt.37@gmail.com; Abbott Abad, Pakistan.

Hummaira Farah, The University of Lahore

Associate Professor; hummaira.farah@sps.uol.edu.pk; Department of Sports Sciences and Physical Education, Faculty of Allied Health Sciences; Lahore, Pakistan.

References

Qin L, Hu S, Xu D, Wang H, Xuan W, Lu T, et al. The Effect of Hip Joint Functional Training on Speed, Flexibility, and Related Performance in Physical Education in College Students. Applied Sciences, 2025;15(20): 11037. https://doi.org/10.3390/app152011037

Tedeschi R, Giorgi F, Donati D. Sprint Training for Hamstring Injury Prevention: A Scoping Review. Applied Sciences, 2025;15(16): 9003. https://doi.org/10.3390/app15169003

Nikolaidis PT, Son’kin VD. Sports Physiology in Adolescent Track-and-Field Athletes: A Narrative Review. Open Access Journal of Sports Medicine, 2023;Volume 14: 59–68. https://doi.org/10.2147/OAJSM.S417612

Myeza N, Mdladla N, Shandu N. Enhancing Performance And Reducing Injury Risk: Integrating Resistance And Flexibility Training For Hamstring Injury Prevention In Rural-based Soccer Players– A Narrative Review. 2024. https://doi.org/10.20944/preprints202405.1669.v1

Herrera E, Osorio-Fuentealba C. Impact of warm-up methods on strength-speed for sprinters in athletics: a mini review. Frontiers in Sports and Active Living, 2024;6: 1360414. https://doi.org/10.3389/fspor.2024.1360414

Bryant J. Effects of high-intensity static stretching on range of motion, performance, muscle activation and architecture [doctoral dissertation]. Worcester: University of Worcester; [cited 2026 Mar 19]. Available from: https://eprints.worc.ac.uk/15744/13/J%20Bryant%20Thesis%20Completed%20_Redacted%20Text%20colour%20edited.pdf

Behm D. Does Stretching Affect Performance? In: The Science and Physiology of Flexibility and Stretching, 2nd edn London: Routledge; 2024. p. 129–160. https://doi.org/10.4324/9781032709086-10

Daneshjoo A, Hosseini E, Heshmati S, Sahebozamani M, Behm DG. Effects of slow dynamic, fast dynamic, and static stretching on recovery of performance, range of motion, balance, and joint position sense in healthy adults. BMC Sports Science, Medicine and Rehabilitation, 2024;16(1): 167. https://doi.org/10.1186/s13102-024-00841-5

Opplert J, Babault N. Acute Effects of Dynamic Stretching on Muscle Flexibility and Performance: An Analysis of the Current Literature. Sports Medicine, 2018;48(2): 299–325. https://doi.org/10.1007/s40279-017-0797-9

Trybulski R, Kużdżał A, Kawczyński A, Klich S, Muracki J, Clemente FM. Postexercise Physical Recovery Methods for Combat Sports: A Scoping Review. International Journal of Sports Medicine, 2026;47(01): 3–23. https://doi.org/10.1055/a-2615-3553

Furrer R, Hawley JA, Handschin C. The molecular athlete: exercise physiology from mechanisms to medals. Physiological Reviews, 2023;103(3): 1693–1787. https://doi.org/10.1152/physrev.00017.2022

Sassone K. Application of proprioceptive neuromuscular facilitation (PNF) to artistic gymnastics [honors thesis]. Baton Rouge: Louisiana State University; 2021 [cited 2026 Mar 19]. Available from: https://repository.lsu.edu/honors_etd/1362

Stańczak M, Swinnen B, Kacprzak B, Pacek A, Surmacz J. Neurophysiology of ACL Injury. Orthopedic Reviews, 2025;17. https://doi.org/10.52965/001c.129173

Toffoli G. Perceived exertion: metrological approaches and applications to endurance performance in able-bodied and spinal cord injured populations [doctoral dissertation]. Bologna: University of Bologna; [cited 2026 Mar 19]. Available from: https://amsdottorato.unibo.it/id/eprint/11138/1/Final_Thesis_Manuscript.pdf

Biscardi LM, Miller AD, Andre MJ, Stroiney DA. Self-efficacy, Effort, and Performance Perceptions Enhance Psychological Responses to Strength Training in National Collegiate Athletic Association Division I Athletes. Journal of Strength & Conditioning Research, 2024;38(5): 898–905. https://doi.org/10.1519/JSC.0000000000004728

Fennell CRJ, Hopker JG. The acute physiological and perceptual effects of recovery interval intensity during cycling-based high-intensity interval training. European Journal of Applied Physiology, 2021;121(2): 425–434. https://doi.org/10.1007/s00421-020-04535-x

Li YT, Zhou Y. Experimental research of impact on psychological state for adolescents with high-intensity interval training intervention. Frontiers in Psychology, 2025;16: 1567003. https://doi.org/10.3389/fpsyg.2025.1567003

Forján‐Barcia A, Hernandez‐Lucas P, Justo‐Cousiño LA. Immediate Effects of Abdominal Hypopressive Technique Versus PNF Stretching in Shortened Hamstring Syndrome: A Pilot Randomized Controlled Trial. Physiotherapy Research International, 2026;31(1): e70155. https://doi.org/10.1002/pri.70155

Liu X, Shao Y, Saha S, Zhao Z, Karmakar D. Maximizing sprint performance among adolescent sprinters: a controlled evaluation of functional, traditional, and combined training approaches. Frontiers in Public Health, 2025;13: 1596381. https://doi.org/10.3389/fpubh.2025.1596381

Renshaw I, Davids K, Araújo D, Lucas A, Roberts WM, Newcombe DJ, et al. Evaluating Weaknesses of “Perceptual-Cognitive Training” and “Brain Training” Methods in Sport: An Ecological Dynamics Critique. Frontiers in Psychology, 2019;9: 2468. https://doi.org/10.3389/fpsyg.2018.02468

Gualtieri A. High-speed running in professional football: monitoring and training in elite adult players [doctoral dissertation]. Norwich: University of East Anglia; 2025 [cited 2026 Mar 19]. Available from: https://ueaeprints.uea.ac.uk/id/eprint/100911/1/2025GualtieriAPhD.pdf

Puentedura EJ, Huijbregts PA, Celeste S, Edwards D, In A, Landers MR, et al. Immediate effects of quantified hamstring stretching: Hold-relax proprioceptive neuromuscular facilitation versus static stretching. Physical Therapy in Sport, 2011;12(3): 122–126. https://doi.org/10.1016/j.ptsp.2011.02.006

Arun B, Anandan D. Impact of proprioceptive neuromuscular facilitation (PNF) techniques on the range of motion (ROM) and quality of life (QOL) in individuals who have undergone ACL ligament reconstruction surgery. Res J Sport Health Psychol. 2024;6(3):153.

Feland B, Hopkins AC, Behm DG. Acute Hemodynamic Responses to Three Types of Hamstrings Stretching in Senior Athletes. Journal of Sports Science and Medicine, 2021; 690–698. https://doi.org/10.52082/jssm.2021.690

Mehraban Jahromi M, Vlček P, Grünerová Lippertová M. Stretching exercises in managing spasticity: effectiveness, risks, and adjunct therapies. European Journal of Translational Myology, 2024; https://doi.org/10.4081/ejtm.2024.12455

Behm DG, Konrad A, Nakamura M, Alizadeh S, Culleton R, Hadjizadeh Anvar S, et al. A narrative review of velocity-based training best practice: the importance of contraction intent versus movement speed. Applied Physiology, Nutrition, and Metabolism, 2025;50: 1–9. https://doi.org/10.1139/apnm-2024-0136

Apte S, Prigent G, Stöggl T, Martínez A, Snyder C, Gremeaux-Bader V, et al. Biomechanical Response of the Lower Extremity to Running-Induced Acute Fatigue: A Systematic Review. Frontiers in Physiology, 2021;12: 646042. https://doi.org/10.3389/fphys.2021.646042

Deguzman L, Flanagan SP, Stecyk S, Montgomery MM. The Immediate Effects of Self-administered Dynamic Warm-up, Proprioceptive Neuromuscular Facilitation, and Foam Rolling on Hamstring Tightness. Athletic Training & Sports Health Care, 2018;10(3): 108–116. https://doi.org/10.3928/19425864-20171101-07

Guo C, Chen F, Wang Y. The Psychological Impact of Competitive Sports Participation on Adolescent Athletes: an Analysis of Coping Mechanisms and Performance Outcomes. Journal of Rational-Emotive & Cognitive-Behavior Therapy, 2025;43(2): 24. https://doi.org/10.1007/s10942-025-00587-0

Zhang P, Chen J, Xing T. Effects of post-exercise stretching versus no stretching on lower limb muscle recovery and performance: a meta-analysis. Frontiers in Physiology, 2025;16: 1674871. https://doi.org/10.3389/fphys.2025.1674871

Bourne M, Schuermans J, Witvrouw E, Aagaard P, Shield A. Neuromuscular Factors Related to Hamstring Muscle Function, Performance and Injury. In: Thorborg K, Opar D, Shield A (eds) Prevention and Rehabilitation of Hamstring Injuries, Cham: Springer International Publishing; 2020. p. 117–143. https://doi.org/10.1007/978-3-030-31638-9_5

Filius SJ, Papa K, Harlaar J. Review of upper extremity passive joint impedance identification in people with Duchenne Muscular Dystrophy. Journal of NeuroEngineering and Rehabilitation, 2025;22(1): 13. https://doi.org/10.1186/s12984-024-01537-0

Pietraszewski P, Maszczyk A, Zając A, Gołaś A. Muscle Activity and Biomechanics of Sprinting: A Meta-Analysis Review. Applied Sciences, 2025;15(9): 4959. https://doi.org/10.3390/app15094959

Rudisill SS, Varady NH, Kucharik MP, Eberlin CT, Martin SD. Evidence-Based Hamstring Injury Prevention and Risk Factor Management: A Systematic Review and Meta-analysis of Randomized Controlled Trials. The American Journal of Sports Medicine, 2023;51(7): 1927–1942. https://doi.org/10.1177/03635465221083998

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Published

2026-03-29

How to Cite

1.
Marwat MK, Iqbal Y, Gillani SMB, Alam M, Salman M, Mehmood K, Khan MA, Farah H. Comparative effects of proprioceptive neuromuscular facilitation hold-relax and dynamic stretching on hamstring flexibility, sprint performance, and perceived exertion in adolescent sprinters. Pedagogy of Physical Culture and Sports. 2026;30(4):306-15.
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