The effect of exercise on homocysteine and some blood parameters in middle-aged sedentary individuals

Keywords: sedentary, exercise, homocysteine, hematocrit, hemoglobin


Background and Study Aim. The objective of this study is to examine the effect of exercise on the blood levels of homocysteine, hemoglobin and hematocrit in middle-aged sedentary individuals. Material and Methods. A total of 24 middle-aged (35-55 years) sedentary individuals (12 females and 12 males) living in Batman province voluntarily participated in this study. Body weight, body mass index, Homocysteine, Hemoglobin and Hematocrit  blood levels were measured before and after a walking exercise scheduled for 6 weeks. Walking exercise was administered 4 days a week for the 6 weeks. Initially, the walking exercises started as 40 min and increased to 60 min towards the end of the program. In the exercises, walking tempo was kept higher than normal and in parallel to the overall health levels of the subjects. The data obtained were then analyzed through the SPSS 25.00 package program. Results. It was determined that there were statistically significant differences in the pretest and posttest parameters of body weight, body mass index, and Homocysteine  values. Additionally, it was determined that, after the 6-week exercise program applied to the female and male participants, there were statistically significant differences between the pretest and posttest parameters of body weight, body mass index, hematocrit, hemoglobin, and homocysteine blood levels. Conclusions. As the conclusion, it was determined that, after the 6-week exercise program applied to a total of 24 participants (12 females and 12 males), there were statistically significant changes in the values of body weight, body mass index, hematocrit, hemoglobin, and homocysteine blood levels. It is suggested for further studies to apply nutrition programs and exercise protocols on young people regularly doing exercise and active athletes in different branches in order to contribute to sports science.


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Author Biographies

Nuri M. Çelik, Batman University; Batman University, School of Physical Education and Sports; Batman, Turkey.
Mehmet Soyal, İstanbul Gelisim University; Istanbul Gelisim University, School of Physical Education and Sports; Istanbul, Turkey


1. Synevo Medical Laboratories Homocysteine-What is it. [Internet]. Synevo; 2015 May [cited 2020 Feb 1] [updated 2019 Jun 15; cited 2020 Feb 1]. Available from:

2. Mayer EL, Jacobsen DW, Robinson K. Homocysteine and coronary athersclerosis. J Am Coll Cardiol. 1996; 27: 517–527.

3. Hayward R, Ruangthai R, Karnilaw P, Chicco A, Strange R, McCarty H, et al. Attenuation of homocysteine-induced endothelial dysfunction by exercise training. Pathophysiology, 2003;9:207–14.

4. Selhub J. Homosisteine metabolism. Annu Rev Nutr. 1999; 19: 217– 246.

5. Prerost MR, Feldman BF, Herbert WG. Homocysteine, Fibrinogen and physical activity in human males with coronary artery disease. Comparative Haematology International. 1999; 9: 25– 30.

6. Lentz SR. Homocysteine and vascular dysfunction. Life Sciences. 1997; 61(13): 1205– 1215.

7. Gaume V, Mougin F, Figard H, Simon-Rigaud ML, N’Guyen UN, Callier J, et al. Physical Training Decreases Total Plasma Homocysteine and Cysteine in Middle-Aged Subjects. Ann Nutr Metab, 2005;49:125–31.

8. Herrmann M, Schorr H, Obeid R, Urhausen A, Scharhag J, Kindermann W, Herrmann W. Homosisteine increases during endurance exercise. Clin Chem Lab Med. 2003; 41(11): 1518– 1524.

9. Chambers JC, Obeid OA, Kooner JS. Physiological increments in plasma Homocysteine induce vascular endothelial dysfunction in normal human subjects. Arteriosclerosis and Thrombosis. 1999; 19: 2922-2927.

10. Mennen LI, de Courcy GP, Guilland J-C, Ducros V, Bertrais S, Nicolas J-P, et al. Homocysteine, cardiovascular disease risk factors, and habitual diet in the French Supplementation with Antioxidant Vitamins and Minerals Study. The American Journal of Clinical Nutrition, 2002;76:1279–89.

11. Bree A, Verschuren WMM, Blom HJ, Kromhout D. Lifstyle factors and plasma homocysteine concentrations in a general population sample. American Journal of Epidemiology. 2001; 154(2): 150–154.

12. Still RA, McDowell IF. ACP Broadsheet No 152: Clinical implications of plasma homocysteine measurement in cardiovascular disease. J Clin Pathol, 1998; 51:183–8.

13. Frosst P, Blom HJ, Milos R, Goyette P, Sheppard CA, Matthews RG, et al. A candidate genetic risk factor for vascular disease: a common mutation in methylenetetrahydrofolate reductase. Nat Genet 1995;10:111–3.

14. Brattstrom L, Wilcken DE, Ohrvik J, Brudin L. Common methylenetetrahydrofolate reductase gene mutation leads to hyperhomocysteinemia but not to vascular disease: the result of a meta-analysis. Circulation, 1998;98:2520–6.

15. Finkelstein J D. The metabolism of homocysteine: pathways and regulation. E ur J Pediatr, 1998; 157 (suppl 2): S40–4.

16. Panagiotakos DB, Pitsavos C, Zcimbckis A, Chrysohoou C, Stefanadis C. The association between lifestly-related factors and plasma homocysteine lebels in healthy individuals from the “ATTICA” study. International Journal of Cardiology. 2004;1: 1–7.

17. Selhub J, Jacques PF, Wilson PW, Rush D, Rosenberg IH. Vitamin status and intake as ın ary determinants of homocysteinemia in an elderly population. JAMA, 1993;270:2693–8.

18. Savage DG, Lindenbaum J, Stabler SP, Alien RH. Sensitivity of serum mcthylmalonic acid and total homocysteine deterrninants for diagnosing cobalamin and foIate deficiencies. Am I Med, 1994;96:239–46.

19. McCully KS. Homocysteine and vascular disease. Nat Med, 1996;2:386–9.

20. Unt E, Zilmer K, Mägi A, Kullisaar T, Kairane C, Zilmer M. Homocysteine status in former top-level male athletes: possible effect of physical activity and physical fitness. Scandinavian Journal of Medicine & Science in Sports, 2008;18:360–6.

21. Joubert LM, Manore MM. Exercise, nutrition, and homocysteine. International Journal of Sport Nutrition and Exercise Metabolism. 2006; 16: 341–361.

22. Real JT, Merchante A, Gomez JL, Chaves FJ. Effects of marathon running on plasma total homocysteine concentrations. Nutr Metab Cardiovasc. 2005; 15: 134–139.

23. König D, Bissé E, Deibert P, Müller H-M, Wieland H, Berg A. Influence of Training Volume and Acute Physical Exercise on the Homocysteine Levels in Endurance-Trained Men: Interactions with Plasma Folate and Vitamin B12. Ann Nutr Metab, 2003;47:114–8.

24. Wright M, Francis K, Cornwell P. Effect of acute exercise on plasma homocysteine. J Sports Med Phys Fitness. 1998;38(3): 262–5.

25. Boone CH, Hoffman JR, Gonzalez AM, Jajtner AR, Townsend JR, Baker KM, et al. Changes in Plasma Aldosterone and Electrolytes Following High-Volume and High-Intensity Resistance Exercise Protocols in Trained Men. Journal of Strength and Conditioning Research, 2016;30:1917–23.

26. Peçanha T, Paula-Ribeiro M, Campana-Rezende E, Bartels R, Marins JCB, de Lima JRP. Water Intake Accelerates Parasympathetic Reactivation After High-Intensity Exercise. International Journal of Sport Nutrition and Exercise Metabolism, 2014;24:489–96.

27. Londeann R. Low heamatcrits during basic training athletes anemia. Nengld J Med, 1978;299: 1191–2.

28. Thorner W. Quoted by srein hus AH. Choronic effects of exercise. Physiol Rew, 1933;24:622– 4.

29. Çavuşoğlu H Egzersiz ve kan. Exercise and blood. İstanbul Tıp Fakültesi 11. Kurultayı Bildiri Kitabı, 249 – 252., Istanbul Medical Faculty 11th Congress Proceedings Book, 1991. P.249 – 252. (In Turkısh)

30. Rousseau AS, Robin S, Roussel AM, Ducros V, Margaritis I. Plasma homocysteine is related to folate intake but not training status. Nutrition Metabolism & Cardiovascular Diseases. 2005; 15: 125–133.

31. Randeva HS, Lewandowski KC, Drzewoski J, Brooke-Wavell K, O’Callaghan C, Czupryniak L, et al. Exercise Decreases Plasma Total Homocysteine in Overweight Young Women with Polycystic Ovary Syndrome. The Journal of Clinical Endocrinology & Metabolism, 2002;87:4496–501.

32. Steenge GR, Verhoef P, Greenhaff PL. The effect of creating and resistance training on plasma homocysteine concentration in healthy volunteers. Archives of Internal Medicine. 2001; 161: 1455–56.

33. Bailey DM, Davies B, Baker J. Training in hypoxia: modulation of metabolic and cardiovascular risk factors in men. Med Sci Sports Exerc. 2000; 32: 1058–1066.

34. Nygard O, Vollset SE, Refsum H, Stensvold I, Tverdal A, Nordrehaug JE, et al. Total plasma homocysteine and cardiovascular risk profile. The Hordaland Homocysteine Study. JAMA: The Journal of the American Medical Association, 1995;274:1526–33.

35. Duncan G, Perri MG, Anton SD, Limacher MC, Martin D, Lowenthal DT, et al. Effects of exercise on emerging and traditional cardiovascular risk factors. Preventive Medicine, 2004;39:894–902.

36. Dinç N. Aerobik egzersizin ve multivitamin kullanımının lipid, homosistein ve antioksidan metabolizması üzerine etkileri. Yüksek Lisans Tezi. [Effects of aerobic exercise and multivitamin use on lipid, homocysteine and antioxidant metabolism]. [Master Thesis]. Celal Bayar University, Institute of Health Sciences; 2006. (In Turkish)

37. Cooper A, Kendrick, A, Stansbie D, Sargent D. Plasma homocysteine in sedentary men: influence of moderately intense exercise. CVR & R. 2000; 21: 371–74.

38. Vincent KR, Braith RW, Bottiglieri T, Heather K, Vincent HK, Lowenthal DT. Homocysteine and lipoprotein levels following resistance training in older adults. Preventive Cardiology. 2003; 6(4): 197–203.

39. De Cree C, Malinow MR, Van Kranenburg GP, Geurten PG, Longford NT, Keizer HA. İnfluence of exercise and menstrual cycle phase on plasma homocysteine levels in young women a prospective study. Scand J Med Sci Sport. 1999; 9: 272–278.

40. Ünal M. Aerobik ve anaerobik akut-kronik egzersizlerin immün parametreler üzerindeki etkileri [Effects of aerobic and anaerobic acute-chronic exercises on immune parameters]. Istanbul; 1998. (In Turkish)

41. Freund BJ, Shizuru EM, Hashiro GM, Claybaugh JR. Hormonal, electrolyte, and renal responses to exercise are intensity dependent. Journal of Applied Physiology, 1991;70:900–6.

42. Nieman DC, Pedersen BK. Exercise And İmmune Function: Recent Development. Sports Med, 1999;27: 73–80.

43. Gallagher PM, Carrithers JA, Godard MP, Schulze KE, Trappe and SW. β-Hydroxy-βMethylbutyrate İngestion, Part II: Effects on Hematology, Hepatic and Renal Function. Medicine and Science in Sports and Exercise, 2000;32:2116–9.

44. Büyükyazı G, Turgay F. Sürekli ve yaygın interval koşu egzersizlerinin bazı hematolojik parametreler üzerine akut ve kronik etkileri [Acute and chronic effects of continuous and widespread interval running exercises on some hematological parameters]. HU Sports Science. And Technic. School VI. Sports Research Congress Paper., 3–5 November, Ankara; 2000. P. 182. ( In Turkish)

45. Halson SL, Lancaster GI, Jeukendrup AE, Gleeson M. Immunological Responses to Overreaching in Cyclists. Medicine & Science in Sports & Exercise, 2003;35:854–61.

46. Ersöz G, Köksoy A, Zergeroğlu AM, Yavuzer S. Akut-Kronik Fiziksel egzersiz ve immunglobulinler. [Chronic Physical exercise and immunoglobulins]. Journal of Sports Sciences, 1995;6 (3): 3–12. (In Turkish)

47. Wade CE, Ramee SR, Hunt MM, While CJ. Hormonal and renal responses to converting enzyme inhibition during maximal exercise. J Appl Physiol, 1987;63: 1796–800.

48. Patlar S. 4 haftalık kronik submaksimal egzersizin lökosit ve lökosit alt grupları üzerindeki etkisi. [The effect of 4-week chronic submaximal exercise on leukocyte and leukocyte subgroups]. 9th International Sports Sciences Congress, Mugla; 2006. P.226–227. (In Turkish)

49. Mashiko T, Umeda T, Nakaji S, Sugawara K. Effects of exercise on the physical condition ofcollege rugby players during summer training camp. Br j Sports Med, 2004;38: 186–190.

50. Gren HJ, Sutton JR, Coates G, Ali M, Jones S. Response of red cell and plasma volume to prolonged training in human. Journal of applied physiology, 1991;70(4): 1810–1815.

51. Su YC, Lin CJ, Chen KT, Lee SM, Lin JS, Tsai CC, et al. Effects of huangqi jianzhong tang on hematological and biochemical parameters in judo athletes. Acta Pharmacol Sin. 2001;22: 1154–8.

52. Rietjens GJ, Kuipers H, Hartgens F, Keizer HA. Red blood cell profile of elite olympic distance triathletes. A three-year follow-up. Int. J. Sports Med, 2002;23(6):391– 25.

53. Davidson RJL, Robertson JD, Gales G and Maughan RJ. Hematological changes associated with marathon running. Int. J. Sports Med. 1987;8:19–25.

54. Dressendorfer RH, Wadle CE and Amsterdam EA. Development of pseudoanemia in marathon runners during a 20-day road race. JAMA, 1981;246:1215–1218.

55. Beamount W. Red cell volume with changes in plasma osmolarity during maximal exercise. Journal apply physiol, 1973;35: 47–50.

56. Berkarda B. Kan Hastalıkları [Blood Diseases]. Istanbul; 2003. ( In Turkish)
How to Cite
Çelik N, Soyal M. The effect of exercise on homocysteine and some blood parameters in middle-aged sedentary individuals. Pedagogy of Physical Culture and Sports. 2020;24(5):219-26.